Adaptation of flocculent anammox culture to low temperature by cold shock: long-term response of the microbial population

Partial nitritation-anammox (PN/A) process will substantially reduce the costs for removal of nitrogen in mainstream municipal sewage. However, one PN/A challenges is to time necessary adaptation anammox bacteria lower temperatures mild climates. In this study, we exposed flocculent culture cold shocks [35°C ? 5°C (8 h) 15°C] and evaluated long-term shock response. Over a post-shock period 40 d at 15°C, rates shocked were significantly higher compared control, with maximum up 0.082 0.033 kg-N/kg-VSS/d or 0.164 0.076 kg-N/m3/d, respectively. corresponding semi-batch cycles, was on average 136 ± 101% more active than due negative effect side populations cells. Per FISH, Ca. Brocadia anammoxidans Scalindua survived remained present throughout. Thus, both microorganisms seem respond favourably shocks. sum, provide further evidence that accelerate WWTPs. Further, first time, report adaptive response